Submitting NGS sequences to NCBI SRA

Backing up raw sequences from Azenta

learning journey
code
Author

Sarah Tanja

Published

October 8, 2024

Modified

September 1, 2025

So you got an email from Azenta saying your files are ready for download! Exciting! It’s the moment we’ve been waiting for. What next??

1 Background research

2 Log in to NCBI

Navigate to the NCBI Submission Portal: https://submit.ncbi.nlm.nih.gov/

Log-in through a partner sign-in, or sign-up directly with NCBI to create an account.

I signed in using my existing ORC-ID account, and authorized NCBI to access my ORCID record.

Using my orcid, I created a new NCBI account.

I was prompted to update my profile and confirm my primary email address in order to be able to submit to SRA.

Once at the Submission portal and logged-in, click on SRA to continue to the Sequence Read Archive (SRA) submission portal: https://submit.ncbi.nlm.nih.gov/about/sra/

Click SUBMIT to start the submission process.

Click New Submission

There are 5 steps to submission:

3 1. Submitter contact info (fill this out)

4 2. General Info

Click CONTINUE

5 3. Project Info

  • Project Title

  • Public description

  • Grants

6 4. Biosample Type

  • Typed in Montipora capitata

  • Under displayed NCBI packages I selected ‘Invertebrate

  • Clicked CONTINUE

7 5. Biosample Attributes

  • Use built-in table editor

    The following are the columns:

  • Sample Name

  • Sample title

8 6. SRA Metadata