Posts
Summary of previous research on microplastic leachate exposure in marine invertebrate embryonic development
In sea urchin and mussel model organisms
learning journey
coral
(jimenez-guri2023?) either exposed adult sea urchins Paracentrotus lividus to excessively high1 levels of PVC leachate or filtered seawater control for 2 weeks, and then force-spawned twice (once at the end of week 1 and once at the end of week 2) where embryos from…
Nikon scope pictures annotation & data collection
Annotating images of embryonic development of Montipora capitata rice corals exposed to PVC leachate
protocols
coral
This guide is meant to be a checklist for annotating categories of embryonic development in Montipora capitata using NIS-Elements BR.
Statistics for embryo scope data
Exploring data from embryonic development of Montipora capitata rice corals exposed to PVC leachate
stats
coral
What information can we get from looking at the Montipora capitata embryos that were exposed to varying levels of PVC leachate (High: 1mg/L, Mid: 0.1mg/L, Low: 0.01mg/L)? This experiment allowed embryos to develop from a bundle-bundle…
Extracting & measuring chlorophyll a & c2 concentrations
… from an anemone-hosted symbiotic dinoflagellate using a ThermoFischer Nanodrop 2000c
protocols
anemones
This protocol draws heavily (and almost exclusively!) from the awesome method paper, “Its What’s on the Inside That Counts: An Effective…
Single stressor leachate exposure summary
A 48 hour exposure to polyvinyl chloride (PVC) leachate and to aged polypropylene (PP) leachate
lab records
This summary serves as a broad overview and notes of the acute (48hour) single stressor polyvinyl chloride (PVC) and polypropylene (PP) leachate exposure to Anthop…
Field collection at Constellation Park, Alki, West Seattle
anemones
lab records
On Saturday, March 30th, 2024 Kip and I collected Anthopleura elegantissima from Constellation Park near Alki in West Seattle, Washington under WDFW scientific collection permit Tanja 24-010. At this site we collected 10 anemones from each of 4…
Leachate preparation & dilution protocol
Modified from the lab protocol of Dr. Francesco Saliu and Valerio Isa
protocols
In order to study the effects of
microplastic leachate we have to be able to re-create it in a controlled and defined way. For this, I partnered with Dr. Francesco Saliu and his lab of environmental chemists…
Master’s Thesis Progress
learning journey
committee
SAFS Masters Thesis Milestones are laid out here
Phthalate exposure to coral embryos methods
M. capitata embryo development and EPA 506 pthalate mix 1 acute exposure experimental methods
lab records
protocols
Plasticizers are a suite of chemicals added to plastic consumer products to increase flexibily. The most widely used plasticizers are phthalate acid esters (PAEs). PAE’s are…
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